Catalog tus lej | RC-CF 31 |
Cov ntsiab lus | Kev kuaj pom ntawm Canine Dirofilaria immitis antigens, Anaplasma antibodies, E. canis antibodies thiab LSH antibodies hauv 10 feeb |
Txoj Cai | Ib-kauj ruam immunochromatographic xeem |
Tshawb Pom Cov Hom Phiaj | CHW Ag : Dirofilaria immitis antigens Anapalsma Ab : Anaplasma antibodiesE. canis Ab : E. canis antibodies LSH Ab : L. chagasi, L. infantum, and L. donovani tshuaj tiv thaiv |
Qauv | Canine Whole Blood, Plasma lossis Serum |
Lub sijhawm nyeem ntawv | 10 feeb |
Ntau | 1 lub thawv (cov khoom siv) = 10 khoom siv (ib tus neeg ntim khoom) |
Cov ntsiab lus | Cov khoom siv kuaj, Buffer fwj, thiab Disposable dropper |
Cia | Chav ntsuas kub (ntawm 2 ~ 30 ℃) |
Kev tas sij hawm | 24 lub hlis tom qab kev tsim khoom |
Ceev faj | Siv li ntawm 10 feeb tom qab qhibSiv cov qauv tsim nyog (0.01 ml ntawm ib lub dropper) Siv tom qab 15 ~ 30 feeb ntawm RT yog tias lawv khaws cia hauv qhov xwm txheej txias Xav txog qhov kev xeem tau ua tsis raug tom qab 10 feeb |
Cov neeg laus lub plawv loj hlob ob peb nti ntev thiab nyob hauv cov hlab ntsha pulmonary uas nws tuaj yeem tau txais cov as-ham txaus.Cov kab mob plawv hauv cov hlab ntsha ua rau mob thiab tsim hematoma.Lub plawv, ces, yuav tsum tau twj tso kua mis ntau dua li ua ntej raws li cov kab mob plawv nce zuj zus, thaiv cov hlab ntsha.
Thaum tus kab mob no deteriorates (tshaj 25 tus kab mob plawv muaj nyob hauv 18 kg aub), cov kab mob plawv txav mus rau hauv txoj cai atrium, thaiv cov ntshav ntws.
Thaum tus naj npawb ntawm cov kab mob plawv mus txog ntau dua 50, lawv tuaj yeem nyob
atriums thiab ventricles.
Thaum kis tau ntau tshaj 100 tus kab mob plawv nyob rau sab xis ntawm lub plawv, tus dev poob lub luag haujlwm ntawm lub plawv thiab nws thiaj li tuag.Qhov no tuag
phenomenon hu ua "Caval Syndrom."
Tsis zoo li lwm cov kab mob, cov kab mob plawv nteg cov kab me me hu ua microfilaria.Microfilaria nyob rau hauv yoov tshaj cum txav mus rau hauv ib tug dev thaum yoov tshaj cum nqus ntshav los ntawm tus dev.Cov kab mob plawv uas tuaj yeem muaj sia nyob hauv tus tswv tsev tau 2 xyoos tuag yog tias lawv tsis txav mus rau lwm tus tswv hauv lub sijhawm ntawd.Cov kab mob uas nyob hauv tus dev cev xeeb tub tuaj yeem kis tau nws lub embryo.
Kev ntsuam xyuas ntxov ntawm cov kab mob plawv yog qhov tseem ceeb heev hauv kev tshem tawm lawv.Heartworms mus dhau ob peb kauj ruam xws li L1, L2, L3 nrog rau theem kis los ntawm yoov tshaj cum los ua tus neeg laus lub plawv.
Microfilaria nyob rau hauv yoov tshaj cum loj hlob mus rau L2 thiab L3 parasites tuaj yeem kis tau rau dev hauv ob peb lub lis piam.Kev loj hlob nyob ntawm huab cua.Qhov kub zoo rau cov kab mob parasite yog tshaj 13.9 ℃.
Thaum tus kab mob yoov tshaj cum tom aub, microfilaria ntawm L3 nkag mus rau hauv nws daim tawv nqaij.Hauv daim tawv nqaij, microfilaria loj hlob mus rau L4 rau 1 ~ 2 lub lis piam.Tom qab nyob hauv daim tawv nqaij rau 3 lub hlis, L4 txhim kho rau hauv L5, uas txav mus rau hauv cov ntshav.
L5 raws li daim ntawv ntawm cov neeg laus cov kab mob plawv nkag mus rau hauv lub plawv thiab cov hlab ntsha pulmonary uas 5 ~ 7 lub hlis tom qab cov kab mob plawv nteg kab.
Cov kab mob keeb kwm thiab cov ntaub ntawv kho mob ntawm tus dev mob, thiab ntau yam kev kuaj mob yuav tsum tau txiav txim siab hauv kev kuaj tus dev.Piv txwv li, X-ray, ultrasound scan, kuaj ntshav, kuaj pom microfilaria thiab, qhov phem tshaj plaws, yuav tsum muaj kev txiav txim siab.
Kev kuaj ntshav;
Kev kuaj xyuas cov tshuaj tiv thaiv kab mob lossis cov tshuaj tiv thaiv kab mob hauv cov ntshav
Antigen kuaj;
Qhov no tsom mus rau kev txheeb xyuas cov antigens tshwj xeeb ntawm poj niam cov neeg laus mob plawv.Kev ntsuam xyuas yog ua nyob rau hauv lub tsev kho mob thiab nws txoj kev vam meej yog siab.Cov khoom siv kuaj muaj nyob rau ntawm lub khw yog tsim los kuaj cov neeg laus hnub nyoog 7 ~ 8 lub hlis kom cov kab mob plawv hnub nyoog qis dua 5 lub hlis tsis yooj yim los kuaj.
Cov kab mob ntawm cov kab mob plawv tau ua tiav zoo hauv feem ntau.Txhawm rau tshem tawm tag nrho cov kab mob plawv, kev siv tshuaj yog qhov zoo tshaj plaws.Kev tshawb pom ntxov ntawm cov kab mob plawv nce qhov kev ua tiav ntawm kev kho mob.Txawm li cas los xij, nyob rau theem kawg ntawm kev kis tus kab mob, qhov teeb meem tuaj yeem tshwm sim, ua rau kev kho mob nyuaj dua.
Cov kab mob Anaplasma phagocytophilum (yav tas los Ehrilichia phagocytophila) tuaj yeem ua rau muaj kab mob hauv ntau hom tsiaj xws li tib neeg.Tus kab mob nyob rau hauv domestic ruminants tseem hu ua zuam-borne fever (TBF), thiab tau paub tsawg kawg yog 200 xyoo.Cov kab mob ntawm tsev neeg Anaplasmataceae yog gram-negative, nonmotile, coccoid rau ellipsoid kab mob, sib txawv ntawm qhov loj ntawm 0.2 mus rau 2.0um txoj kab uas hla.Lawv yog obligate aerobes, tsis muaj txoj hauv kev glycolytic, thiab tag nrho yog cov kab mob hauv lub cev.Tag nrho cov tsiaj nyob rau hauv genus Anaplasma nyob rau hauv daim nyias nyias-lined vacuoles nyob rau hauv tsis paub tab los yog paub tab hematopoietic hlwb ntawm mammalian party.Ib tug phagocytophilum kis neutrophils thiab lo lus granulocytotropic yog hais txog tus kab mob neutrophils.Tsis tshua muaj kab mob, tau pom nyob rau hauv eosinophils.
Anaplasma phagocytophilum
Cov tsos mob tshwm sim ntawmCanine anaplasmosis muaj xws li kub taub hau, lethargy, kev nyuaj siab thiab polyarthritis.Cov tsos mob ntawm neurologic (ataxia, qaug dab peg thiab mob caj dab) kuj tuaj yeem pom.Tus kab mob Anaplasma phagocytophilum tsis tshua muaj neeg tuag tshwj tsis yog muaj teeb meem los ntawm lwm yam kab mob.Kev poob ncaj qha, kev poob qis thiab kev tsim khoom poob tau raug pom nyob hauv cov menyuam yaj.Abortion thiab impaired spermatogenesis nyob rau hauv yaj thiab nyuj tau sau tseg.Qhov mob hnyav ntawm tus kab mob yog cuam tshuam los ntawm ntau yam, xws li kev hloov pauv ntawm Anaplasma phagocytophilum koom nrog, lwm yam kab mob, hnub nyoog, kev tiv thaiv kab mob thiab kev mob ntawm tus tswv tsev, thiab lwm yam xws li kev nyab xeeb thiab kev tswj hwm.Nws yuav tsum tau hais tias kev kho mob tshwm sim nyob rau hauv tib neeg muaj xws li los ntawm ib tug me me self-limited mob khaub thuas-zoo li tus kab mob, mus rau ib tug kab mob ua rau lub neej.Txawm li cas los xij, feem ntau tib neeg kab mob tej zaum yuav ua rau me me los yog tsis muaj kev kho mob tshwm sim.
Anaplasma phagocytophilum kis tau los ntawm ixodid zuam.Hauv Tebchaws Meskas cov vectors tseem ceeb yog Ixodes scapularis thiab Ixodes pacificus, thaum Ixode ricinus tau pom tias yog lub ntsiab exophilic vector hauv Europe.Anaplasma phagocytophilum yog transstadially kis los ntawm cov zuam vector, thiab tsis muaj pov thawj ntawm transovarial kis.Feem ntau cov kev tshawb fawb txog niaj hnub no tau tshawb xyuas qhov tseem ceeb ntawm cov tsiaj nyeg ntawm A. phagocytophilum thiab nws cov zuam vectors tau tsom mus rau cov nas tab sis cov kab mob no muaj ntau hom tsiaj, kis rau cov miv, dev, yaj, nyuj, thiab nees.
Indirect immunofluorescence assay yog qhov kev sim thawj zaug uas siv los txheeb xyuas tus kab mob.Cov kev kuaj mob hnyav thiab convalescent theem tuaj yeem raug soj ntsuam los nrhiav kev hloov plaub zaug hauv cov tshuaj tiv thaiv kab mob rau Anaplasma phagocytophilum.Intracellular inclusions (morulea) yog pom nyob rau hauv granulocytes ntawm Wright los yog Gimsa stained ntshav smears.Polymerase saw cov tshuaj tiv thaiv (PCR) txoj kev yog siv los ntes Anaplasma phagocytophilum DNA.
Tsis muaj tshuaj txhaj tiv thaiv kab mob Anaplasma phagocytophilum.Kev tiv thaiv cia siab rau kev tiv thaiv kab mob rau cov zuam vector (Ixodes scapularis, Ixodes pacificus, thiab Ixode ricinus) los ntawm lub caij nplooj ntoos hlav mus txog rau lub caij nplooj zeeg, kev siv tshuaj tiv thaiv kab mob, thiab kev siv tshuaj tiv thaiv ntawm doxycycline los yog tetracycline thaum mus xyuas Ixodes scapularis, Ixodes Ixodes pacificus thiab prophylactic. thaj chaw muaj kab mob.
Ehrlichia canis yog ib tug me me thiab pas nrig zoo li tus kab mob kis los ntawm tus aub xim av zuam, Rhipicephalus sanguineus.E. canis yog qhov ua rau classical ehrlichiosis hauv dev.Cov dev yuav kis tau los ntawm ob peb Ehrlichia spp.Tab sis qhov feem ntau ua rau tus kab mob ehrlichiosis yog E. canis.
E. canis tam sim no tau paub tias tau kis thoob plaws hauv Tebchaws Meskas, Tebchaws Europe, South America, Asia thiab Mediterranean.
Cov kab mob dev uas tsis kho tuaj yeem dhau los ua tus neeg muaj tus kab mob asymptomatic rau xyoo thiab nws thiaj li tuag los ntawm hemorrhage loj.
Ehrlichia canis kab mob hauv dev tau muab faib ua 3 theem;
ACUTE PHASE: Qhov no feem ntau yog ib theem me me heev.Tus dev yuav tsis muaj npe, tawm ntawm zaub mov, thiab tej zaum yuav muaj cov qog ntshav loj.Tej zaum yuav ua npaws ib yam nkaus tab sis tsis tshua muaj theem no tua tus dev.Feem ntau tshem cov kab mob ntawm lawv tus kheej tab sis qee qhov yuav mus rau theem tom ntej.
SUBCLINICAL PHASE: Nyob rau theem no, tus dev zoo li qub.Lub cev muaj sequestered nyob rau hauv tus po thiab yog qhov tseem ceeb nkaum tawm muaj.
CHRONIC PHASE: Nyob rau theem no tus dev tau mob dua.Txog li 60% ntawm cov dev uas kis tus kab mob E. canis yuav muaj cov ntshav txawv txav vim txo cov ntshav platelets.Qhov mob tob hauv qhov muag hu ua "uveitis" tuaj yeem tshwm sim los ntawm kev tiv thaiv lub cev mus ntev.Neurological teebmeem kuj yuav pom.
Kev kuaj pom tseeb ntawm Ehrlichia canis yuav tsum pom qhov pom ntawm morula nyob rau hauv monocytes ntawm cytology, kuaj pom E. canis cov tshuaj tiv thaiv kab mob nrog cov tshuaj tiv thaiv kab mob tsis ncaj ncees lawm (IFA), polymerase chain reaction (PCR) amplification, thiab / lossis gel blotting (Western immunoblotting).
Lub ntsiab tseem ceeb ntawm kev tiv thaiv ntawm canine ehrlichiosis yog zuam tswj.Cov tshuaj ntawm kev xaiv rau kev kho mob rau txhua hom ehrlichiosis yog doxycycline rau tsawg kawg ib hlis.Yuav tsum muaj kev txhim kho kev kho mob zoo nyob rau hauv 24-48 teev tom qab pib kho cov dev uas muaj mob hnyav lossis mob me me.Lub sijhawm no, platelet suav pib nce thiab yuav tsum zoo li qub hauv 14 hnub tom qab pib kho.
Tom qab kis tus kab mob, nws muaj peev xwm rov kis tau tus kab mob;kev tiv thaiv kab mob tsis kav ntev tom qab kis kab mob yav dhau los.
Kev tiv thaiv zoo tshaj plaws ntawm ehrlichiosis yog ua kom cov dev tsis muaj zuam.Qhov no yuav tsum suav nrog kuaj xyuas daim tawv nqaij txhua hnub rau zuam thiab kho cov dev nrog zuam tswj.Txij li cov zuam muaj lwm yam kab mob loj heev, xws li kab mob Lyme, anaplasmosis thiab Rocky Mountain pom kub taub hau, nws yog ib qho tseem ceeb kom tsis txhob zuam dev.
Leishmaniasis yog ib yam kab mob loj thiab hnyav heev ntawm tib neeg, canines thiab felines.Tus neeg sawv cev ntawm leishmaniasis yog tus kab mob protozoan thiab belongs rau leishmania donovani complex.Cov kab mob no tau nthuav dav hauv cov teb chaws sov thiab thaj chaw sov ntawm Southern Europe, Africa, Asia, South America thiab Central America.Leishmania donovani infantum (L. infantum) yog lub luag haujlwm rau tus kab mob feline thiab canine nyob rau yav qab teb Europe, Africa, thiab Asia.Canine Leishmaniasis yog ib hom kab mob loj heev.Tsis yog txhua tus dev tsim kab mob tom qab inoculation nrog parasites.Txoj kev loj hlob ntawm kev kho mob yog nyob ntawm seb hom kev tiv thaiv kab mob uas ib tus tsiaj muaj
tiv thaiv kab mob.
Hauv Canine
Ob leeg visceral thiab cutaneous manifestations yuav pom ib txhij nyob rau hauv dev;Tsis zoo li tib neeg, cais cutaneous thiab visceral syndromes tsis pom.Cov tsos mob tshwm sim kuj sib txawv thiab tuaj yeem ua rau lwm yam kab mob.Cov kab mob asymptomatic kuj tuaj yeem tshwm sim.Cov tsos mob ntawm visceral tej zaum yuav muaj xws li kub taub hau (uas tuaj yeem cuam tshuam), mob ntshav qab zib, lymphadenopathy, splenomegaly, lethargy, txo qis kev ua siab ntev, poob phaus, thiab tsis qab los noj mov.Cov tsos mob tshwm sim tsawg dua suav nrog raws plab, ntuav, melena, glomerulonephritis, daim siab tsis ua haujlwm, epistaxis, polyuria-polydipsia, txham, lameness (vim polyarthritis lossis myositis), ascites, thiab mob plab.
Hauv Feline
Miv tsis tshua muaj kab mob.Hauv cov miv uas muaj kab mob feem ntau, cov kab mob tsuas yog siv rau cov kab mob ntawm daim tawv nqaij, feem ntau pom ntawm daim di ncauj, qhov ntswg, daim tawv muag, lossis pinnae.Visceral lesions thiab cov cim qhia tsis tshua muaj.
Lub neej voj voog yog ua tiav nyob rau hauv ob lub tswv yim.Tus tswv tsev vertebrate thiab tus tswv tsev invertebrate (sand fly).Tus poj niam xuab zeb ya pub rau tus tswv tsev vertebrate thiabnoj amastigotes.Flagellated promastigotes tsim nyob rau hauv kab.Cov promastigotes tau txhaj rau hauv lub vertebrate party thaum pub mis nyuj.Cov promastigotes loj hlob mus rau hauv amastigotes thiab sib faib feem ntau hauv macrophages.Multiplication nyob rau hauv lub macrophages ntawm daim tawv nqaij, mucosa thiab viscera, ua rau cutaneous, mucosal thiab visceral leishmaniasis feem.
Hauv cov dev, leishmaniasis feem ntau yog kuaj pom los ntawm kev soj ntsuam ncaj qha ntawm cov kab mob, siv Giemsa lossis cov stains sai sai, hauv smears los ntawm cov qog nqaij hlav, spleen, lossis pob txha pob txha aspirates, cov ntaub so ntswg biopsies, los yog daim tawv nqaij scrapings los ntawm qhov txhab.Cov kab mob kuj tuaj yeem pom nyob rau hauv cov kab mob ntawm qhov muag, tshwj xeeb tshaj yog nyob rau hauv granulomas.Cov amastigotes yog puag ncig mus rau oval parasites, nrog ib puag ncig basophilic nucleus thiab me me rod zoo li kinetoplast.Lawv muaj nyob rau hauv macrophages los yog tso tawm los ntawm ruptured hlwb.Immunohistochemistry thiab polymerase saw tshuaj tiv thaiv (PCR) cov txheej txheem kuj tseem siv.
Cov tshuaj feem ntau siv yog: Meglumine Antimoniate txuam nrog Allopurinol, Aminosidine, thiab tsis ntev los no, Amphotericin B. Tag nrho cov tshuaj no yuav tsum tau siv ntau yam tshuaj, thiab qhov no yuav nyob ntawm tus neeg mob tus mob thiab kev koom tes ntawm tus tswv.Nws raug pom zoo tias kev kho mob yuav tsum tau khaws cia nrog allopurinol, vim tias nws tsis tuaj yeem ua kom ntseeg tau tias cov dev yuav tsis rov qab los yog tias tsis kho.Kev siv cov dab tshos uas muaj tshuaj tua kab, tshuaj zawv plaub hau lossis tshuaj tsuag kom zoo los tiv thaiv cov dev los ntawm cov xuab zeb tom yuav tsum tau siv tsis tu ncua rau txhua tus neeg mob hauv kev kho mob.Kev tswj vector yog ib qho tseem ceeb tshaj plaws ntawm kev tswj kab mob.
Sandfly yog qhov yooj yim rau tib yam tshuaj tua kab raws li tus kab mob malaria vector.