Cov ntsiab lus | Kev txheeb xyuas cov tshuaj tiv thaiv tshwj xeeb ntawm Peste Des Petits Ruminants |
Txoj Cai | PPRV cov tshuaj tiv thaiv kab mob ELISA siv los kuaj xyuas Peste des petits ruminants virus antibodies hauv cov ntshav ntawm yaj thiab tshis. |
Tshawb Pom Cov Hom Phiaj | PPRV cov tshuaj tiv thaiv |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Ovine rinderpest, tseem hu uapeste des petits ruminants(PPR), yog kab mob sib kis feem ntau cuam tshuamtshisthiabyaj;txawm li cas los xij, ntxhuav thiab qus me mecov ruminantskuj tuaj yeem cuam tshuam.PPR tam sim no nyob rau hauvNorth, Central, Sab hnub poobthiabEast Africa, covMiddle East, thiabSouth Asia. Nws yog tshwm sim los ntawmme me ruminants morbillivirushauv genusMorbillivirus,thiab yog ze ze rau, ntawm lwm tus, tus kab mob morbillivirus, mob qhua pias morbillivirus, thiabkab mob morbillivirus(yav dhau los hu uaCaninetus kab mob distemper).Tus kab mob no kis tau heev, thiab muaj peev xwm muaj 80-100% kev tuag nyob rau hauvmob hnyavcas hauv ibepizootickev teeb tsa.Tus kab mob no tsis kis rau tib neeg.
PPR tseem hu ua tshis plague,kata, syndrome ntawm stomatitis-pneumoenteritis, thiab ovine rinderpest.
Cov koom haum nom tswv xws li covFAOthiabOIEsiv lub npe Fabkis "peste des petits ruminants"nrog ntau yam kev sau ntawv.
Cov khoom siv no siv txoj kev sib tw ELISA rau pre-coated PPRV antigens ntawm microplate qhov dej.Thaum kuaj, ntxiv diluted serum qauv, tom qab incubation, yog tias muaj PPRV antibody, nws yuav ua ke nrog pre-coated antigen, antibody hauv cov qauv thaiv kev sib xyaw ntawm monoclonal antibody thiab pre-coated antigen;pov tseg lub uncombined enzyme conjugate nrog ntxuav;Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub teeb xiav xiav los ntawm Enzyme catalysis yog inverse proportion of antibody content in sample.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |