Cov ntsiab lus | Kev kuaj xyuas cov tshuaj tiv thaiv tshwj xeeb ntawm Newcastle cov kab mob tshwj xeeb cov tshuaj tiv thaiv |
Txoj Cai | Newcastle kab mob tshuaj tiv thaiv kab mob Elisa cov khoom siv los kuaj xyuas cov tshuaj tiv thaiv kab mob Newcastle Tus kab mob (NDV) hauv cov ntshav, rau kev saib xyuas cov tshuaj tiv thaiv tom qab NDV tiv thaiv kab mobthiab serological kuaj kab mob hauv Avian.
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Tshawb Pom Cov Hom Phiaj | Newcastle kab mob antibody |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Newcastle tus kab mob yog kab mob kis kab mob avian cuam tshuam rau ntau hom noog hauv tsev thiab tsiaj qus;Nws kis tau mus rau tib neeg.Txawm hais tias nws tuaj yeem kis tau rau tib neeg, feem ntau tsis muaj tsos mob;Tsis tshua muaj nws tuaj yeem ua rau kub taub hau me me thiab cov tsos mob zoo li mob khaub thuas thiab / lossis kab mob conjunctivitis hauv tib neeg.Nws cov teebmeem yog qhov tseem ceeb tshaj plaws hauv cov nqaij qaib hauv tsev vim lawv qhov kev raug mob siab thiab muaj peev xwm ua rau muaj kev cuam tshuam loj ntawm epizootic rau cov nqaij qaib industries.Nws yog endemic rau ntau lub teb chaws.Tsis muaj kev kho mob rau nws paub, tab sis kev siv tshuaj tiv thaiv prophylactic thiab kev ntsuas huv kom txo tau qhov tshwm sim ntawm kev sib kis.
Cov khoom siv no siv thaiv ELISA txoj kev, NDV antigen yog pre-coated ntawm microplate.Thaum kuaj, ntxiv cov kua dej diluted, tom qab incubation, yog tias muaj NDV cov tshuaj tiv thaiv tshwj xeeb, nws yuav ua ke nrog pre-coated antigen, muab pov tseg cov tshuaj tiv thaiv tsis sib xyaw thiab lwm yam khoom siv ntxuav;tom qab ntawd ntxiv enzyme labled anti-NDV monoclonal antibody, antibody hauv cov qauv thaiv kev sib xyaw ntawm monoclonal antibody thiab pre-coated antigen;pov tseg cov enzyme uncombined conjugate nrog ntxuav. Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub teeb xiav xiav los ntawm Enzyme catalysis yog inverse proportion of antibody content in sample.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2.0ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |