Cov ntsiab lus | Kev kuaj xyuas tshwj xeeb ntawm Antibody of Flu Kev tiv thaiv kab mob thiab kev kuaj mob serological ntawm tus kab mob hauv Avian, swine thiab Equus. |
Txoj Cai | Influenza A antibody Elisa cov khoom siv los kuaj xyuas cov tshuaj tiv thaiv tshwj xeeb tiv thaiv Influenza A Virus (Flu A) hauv cov ntshav, txhawm rau saib xyuas cov tshuaj tiv thaiv tom qab Flu Lub cev tsis muaj zogthiab serological kuaj kab mob hauv Avian, swine thiab Equus.
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Tshawb Pom Cov Hom Phiaj | Influenza A antibody |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Influenza A virus yog ib yam kab mob uas ua rau mob khaub thuas hauv cov noog thiab qee cov tsiaj.Nws yog tus kab mob RNA, subtypes uas tau raug cais tawm ntawm cov noog qus.Qee lub sij hawm, nws kis tau los ntawm cov noog qus mus rau cov nqaij qaib, uas tuaj yeem ua rau muaj kab mob hnyav, kis kab mob, lossis mob khaub thuas tib neeg.
Cov khoom siv no siv thaiv ELISA txoj kev, FluA antigen yog pre-coated ntawm microplate.Thaum kuaj, ntxiv cov kua dej ntshiab, tom qab incubation, yog tias muaj mob khaub thuas Ib qho tshuaj tiv thaiv tshwj xeeb, nws yuav ua ke nrog cov tshuaj tiv thaiv ua ntej-coated, pov tseg cov tshuaj tiv thaiv tsis sib xyaw thiab lwm yam khoom siv ntxuav;tom qab ntawd ntxiv enzyme Labled anti-Flu Monoclonal antibody, cov tshuaj tiv thaiv kab mob hauv cov qauv thaiv kev sib xyaw ntawm monoclonal antibody thiab pre-coated antigen;pov tseg cov enzyme uncombined conjugate nrog ntxuav. Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub teeb xiav xiav los ntawm Enzyme catalysis yog inverse proportion of antibody content in sample.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2.0ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |