Cov ntsiab lus | Kev txheeb xyuas qhov tshwj xeebKo taw thiab qhov ncauj kab mob hom A tshuaj tiv thaiv |
Txoj Cai | FMD Hom A tshuaj tiv thaiv kab mob ELISA siv los kuaj xyuas tus kab mob ko taw thiab qhov ncauj kab mob cov tshuaj tiv thaiv kab mob hauv cov ntshav ntawm npua, nyuj, yaj thiab tshis rau kev ntsuas FMD tshuaj tiv thaiv kab mob. |
Tshawb Pom Cov Hom Phiaj | Ko taw thiab qhov ncauj kab mob Hom A Antibody |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Tus kab mob ko taw thiab qhov ncauj(FMDV) yog tuskab mobuas ua raukab mob ko taw thiab qhov ncauj. Nws yog ibtus kab mob piccornavirus, tus prototypical tswv cuab ntawm genusAphthovirus.Tus kab mob, uas ua rau vesicles (blisters) nyob rau hauv lub qhov ncauj thiab ko taw ntawmnyuj, npua, yaj, tshis, thiab lwm yamcloven-hoofedtsiaj yog kis tau tus kab mob loj heevtsiaj ua liaj ua teb.
Cov khoom siv no siv txoj kev sib tw ELISA los ua ntej-coated kab mob ko taw thiab qhov ncauj kab mob antigens ntawm microplate qhov dej.Thaum kuaj, ntxiv diluted serum qauv thiab anti-FMD Ab, tom qab incubation, yog tias muaj FMD antibody, nws yuav ua ke nrog pre-coated antigen, antibody nyob rau hauv cov qauv thaiv kev sib xyaw ntawm anti-FMD antibody thiab pre-coated antigen;pov tseg lub uncombined enzyme conjugate nrog ntxuav;Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub teeb xiav xiav los ntawm Enzyme catalysis yog inverse proportion of antibody content in sample.
Tus kab mob ko taw thiab qhov ncaujtshwm sim nyob rau hauv xya lojserotypes: O, A, C, SAT-1, SAT-2, SAT-3, thiab Asia-1.Cov serotypes no qhia qee thaj tsam, thiab O serotype feem ntau yog.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2.0ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |