Cov khoom
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
| Cov ntsiab lus | Use kom kuaj tau cov tshuaj tiv thaiv tshwj xeeb tiv thaiv EDS76 hauv cov ntshav zoo. |
| Txoj Cai | Cov Eggs Drop Syndrome 1976 virus (EDS76) Ab Elisa cov khoom siv yog siv los kuaj xyuas cov tshuaj tiv thaiv tshwj xeeb tiv thaiv EDS76 hauv cov ntshav zoo.Rau kev saib xyuas cov tshuaj tiv thaiv tom qab EDS76 tiv thaiv kab mob thiab kev kuaj mob serological ntawm tus kab mob hauv Avian. |
| Tshawb Pom Cov Hom Phiaj | Antibody tiv thaiv EDS76 hauv cov ntshav |
| Qauv | Serum
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| Ntau | 1 kitab = 192 test |
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Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Cov ntaub ntawv
Egg Drop Syndrome (EDS-76) yog ib qho kab mob kis tau zoo los ntawm adenovirus pawg III ntawm Adenoviridae Avian Virus genus nrog hemagglutination.Hauv qee qhov nqaij qaib ua liaj ua teb, cov qe qe loj ntawm cov qaib dheev poob qis, thiab cov qe deformed xws li cov qe mos, cov qe qes, thiab cov qe nyias nyias tau tsim tib lub sijhawm.Tag nrho cov kab mob no kav ntev li 5-6 lub lis piam, tom qab ntawd cov qe ntau lawm maj mam nce, tab sis nws yog ib qho nyuaj rau mus txog qib ua ntej poob.
Txoj Cai ntawm Kev Xeem
Cov khoom siv no siv txoj kev tsis ncaj ELISA, pureed EDS76 antigen yog pre-coated ntawm enzyme micro-zoo strips.Whentesting, adddilutedserumsample, afterincubation,yog tias muaj EDS76 tus kab mob tshwj xeeb, nws yuav ua ke nrog pre-coatedantigen, discardtheuncombined antibody thiab lwm yam khoom siv ntxuav;tom qab ntawd ntxiv enzyme labled anti-EDS76 tus kab mob monoclonal antibody, tom qab ntawd ua ke ntawm monoclonal antibody thiab pre-coated antigen;pov tseg lub uncombined enzyme conjugate nrog ntxuav;Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub xiav teeb liab los ntawm Enzyme catalysis yog inverse proportion of antibody cov ntsiab lus nyob rau hauv cov qauv, siv ELISA nyeem ntawv ntawm 450nm wavelenth los ntsuas lub absorbance ib tug nqi nyob rau hauv cov tshuaj tiv thaiv qhov dej tom qab ntxiv cov tshuaj nres.
txwv tsis pub cov tshuaj tiv thaiv.
Cov ntsiab lus
| Reagent | Ntim 96 xeem/192 xeem | ||
| 1 |
| 1ea/2 ua | |
| 2 |
| 2.0ml ib | |
| 3 |
| 1.6ml ib | |
| 4 |
| 100 ml | |
| 5 |
| 100 ml | |
| 6 |
| 1 1/22 ml | |
| 7 |
| 1 1/22 ml | |
| 8 |
| 15ml ib | |
| 9 |
| 2 ua/4ea | |
| 10 | Serum dilution microplate | 1ea/2 ua | |
| 11 | Kev qhia | 1 pcs |
