Cov ntsiab lus | Kev tshawb pom ntawm neutralizing antibody tiv thaiv kab mob bursa ntawm Fabricius tus kab mob hauv qaib cov ntshav |
Tshawb Pom Cov Hom Phiaj | Qaib kis kab mob bursal virus antibody |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Kab mob bursal(IBD), tseem hu ua Gumboro kab mob, kis kab mob bursitis thiab kis avian nephrosis, yog ib tus kab mob kis tau zoo heev ntawm cov tub ntxhais hluas.qaibthiab qaib cov txwv los ntawm tus kab mob kis kab mob bursal (IBDV), yam ntxwv los ntawmkev tiv thaiv kab mobthiab kev tuag feem ntau ntawm 3 mus rau 6 lub lis piam ntawm hnub nyoog.Tus kab mob tau pom thawj zaug hauvGumboro, Delawarexyoo 1962. Nws yog ib qho tseem ceeb rau kev lag luam nqaij qaib thoob ntiaj teb vim muaj kev cuam tshuam rau lwm yam kab mob thiab kev cuam tshuam tsis zoo rau kev ua haujlwm zoo.txhaj tshuaj tiv thaiv.Nyob rau hauv xyoo tas los no, cov kab mob phem heev ntawm IBDV (vvIBDV), ua rau cov nqaij qaib tuag hnyav heev, tau tshwm sim hauv Tebchaws Europe,Latin America,South-East Asia, Africa thiabMiddle East.Kev kis kab mob yog los ntawm txoj kev oro-fecal, nrog rau cov noog uas cuam tshuam rau cov kab mob siab rau kwv yees li 2 lub lis piam tom qab kis kab mob.Tus kab mob no kis tau yooj yim los ntawm cov qaib mus rau cov qaib noj qab haus huv los ntawm kev noj zaub mov, dej, thiab kev sib cuag ntawm lub cev.
Cov khoom siv siv txoj kev sib tw ELISA, pre-packaged infectious bursal disease virus VP2 protein on the microplate, thiab sib tw nrog anti-VP2 protein antibody nyob rau hauv cov ntshav rau cov khoom theem vector siv anti-VP2 protein monoclonal antibody.Hauv kev sim, ib qho tshuaj tiv thaiv kab mob monoclonal yuav tsum tau kuaj thiab ntxiv cov tshuaj tiv thaiv VP2 protein, thiab tom qab incubation, yog tias cov qauv muaj cov kab mob nqaij qaib kis kab mob VP2 cov tshuaj tiv thaiv tshwj xeeb, nws khi rau cov antigen ntawm lub phaj coated.Yog li thaiv kev khi ntawm anti-VP2 protein monoclonal antibody rau cov antigen, tom qab ntxuav kom tshem tawm cov tshuaj tiv thaiv tsis muaj zog thiab lwm yam khoom siv;tom qab ntawd ntxiv cov tshuaj tiv thaiv-nas enzyme-labeled Secondary antibody rau tshwj xeeb khi rau cov antigen-antibody complex ntawm lub phaj kuaj pom;Lub unbound enzyme conjugate raug tshem tawm los ntawm kev ntxuav;TMB substrate yog ntxiv rau lub microwell los tsim cov xim, thiab qhov absorbance tus nqi ntawm cov qauv yog tsis zoo correlated nrog cov ntsiab lus ntawm anti-VP2 protein antibody muaj nyob rau hauv, yog li ua tiav lub hom phiaj ntawm kev soj ntsuam cov anti-VP2 protein antibody nyob rau hauv cov qauv.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2.0ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |