Cov ntsiab lus | Kev kuaj pom tus kab mob tshwj xeeb ntawm Ko taw thiab qhov ncauj kab mob Hom BRU |
Txoj Cai | Cov khoom siv tshuaj tiv thaiv BRU ELISA siv los kuaj xyuas cov kab mob Brucellosis hauv cov ntshav ntawm npua, nyuj, yaj thiab tshis. |
Tshawb Pom Cov Hom Phiaj | BRU tshuaj tiv thaiv |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Lub BRU tshuaj tiv thaivELISAxeem kitsiv rau Kev kuaj pom ntawm Brucellosis cov tshuaj tiv thaiv kab mob hauv cov ntshav ntawm npua, nyuj, spab thiab tshis .
Cov khoom siv no siv kev sib tw ELISA txoj kev ua ntej-coated BRU antigens ntawm microplate qhov dej.Thaum kuaj, ntxiv diluted serum qauv thiabenzyme Labled los tiv thaivBRU monoclonal antibody, tom qab incubation, yog muaj muaj BRU antibody, nws yuav ua ke nrog pre-coated antigen, antibody hauv cov qauv thaiv kev sib xyaw ntawm monoclonal antibody thiab pre-coated antigen;pov tseg lub uncombined enzyme conjugate nrog ntxuav;Ntxiv TMB substrate nyob rau hauv micro-qhov dej, lub teeb xiav xiav los ntawm Enzyme catalysis yog inverse proportion of antibody cov ntsiab lus hauv cov qauv.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2ml ib | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml ib | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |