Cov ntsiab lus | siv los kuaj Avian leukosis P27 antigen hauv avian ntshav, quav, cloaca, thiab qe dawb. |
Txoj Cai | Avian Leukosis (AL) P27 antigen Elisa cov khoom siv los kuaj Avian leukosis P27 antigen hauv avian ntshav, quav, cloaca, thiab qe dawb.
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Tshawb Pom Cov Hom Phiaj | Avian Leukosis (AL) P27 antigen |
Qauv | Serum
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Ntau | 1 kitab = 192 test |
Stability thiab Cia | 1) Tag nrho cov reagents yuav tsum khaws cia ntawm 2 ~ 8 ℃.Tsis txhob khov. 2) Lub neej txee yog 12 lub hlis.Siv tag nrho cov reagents ua ntej hnub tas sij hawm ntawm cov khoom siv.
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Avian Leukosis (AL) yog ib lo lus sib sau ua ke rau ntau yam mob qog nqaij hlav hauv cov nqaij qaib los ntawm Avian Leukosis Virus (ALV) hauv tsev neeg Retroviridae.Tus kab mob no tau muab faib thoob ntiaj teb thiab muaj tus kab mob siab.Nws tuaj yeem ua rau tuag thiab emaciation nyob rau hauv qaib, txo lub peev xwm ntawm cov pab yaj, thiab yog ib qho ntawm cov kab mob tseem ceeb uas ua rau muaj kev phom sij loj rau kev lag luam nqaij qaib.Tus kab mob no muaj keeb kwm ntev thiab tau ntsib cov neeg mob tshiab tas li, xws li Avian leukemia virus subgroup J (ALV-J), uas tau tshawb pom thiab txheeb xyuas thaum xyoo 1980s hauv tebchaws Askiv raws li cov kab mob qog ntshav qog ntshav tshiab, ua rau muaj txiaj ntsig zoo. ua mob rau kev lag luam broiler
Cov khoom siv siv cov txheej txheem ELISA sandwich, pured anti-avian leukocyte P27 monoclonal antibody yog pre-coated ntawm enzyme micro-zoo strips.Nyob rau hauv qhov kev ntsuam xyuas, cov antigen hauv cov qauv yog khi rau cov tshuaj tiv thaiv ntawm lub phaj coated, tom qab ntxuav kom tshem tawm. lub unbound antigen thiab lwm yam Cheebtsam, enzyme monoclonal antibody yog ntxiv rau tshwj xeeb khi rau cov antigen-antibody complex ntawm lub phaj xeem.Tom qab ntawd ntxuav, lub unbound enzyme conjugate raug tshem tawm, TMB substrate tov yog ntxiv rau lub microplate, lub xiav teeb liab los ntawm Enzyme catalysis yog ncaj qha feem ntawm cov tshuaj tiv thaiv cov ntsiab lus hauv cov qauv.Ntxiv cov tshuaj nres, Tom qab cov tshuaj tiv thaiv, qhov absorbance A tus nqi hauv cov tshuaj tiv thaiv zoo yog ntsuas los ntawm lub wavelength ntawm 450 nm.
Reagent | Ntim 96 xeem/192 xeem | ||
1 |
| 1ea/2 ua | |
2 |
| 2.0ml | |
3 |
| 1.6ml ib | |
4 |
| 100 ml | |
5 |
| 100 ml | |
6 |
| 1 1/22 ml | |
7 |
| 1 1/22 ml | |
8 |
| 15ml | |
9 |
| 2 ua/4ea | |
10 | Serum dilution microplate | 1ea/2 ua | |
11 | Kev qhia | 1 pcs |